portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;"> 成分：
portant; word-wrap: break-word !important; font-family: 宋体;"> 牛肉膏 5g
portant; word-wrap: break-word !important;">portant; word-wrap: break-word !important;">蛋白胨 10g蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 3g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钠 2g
0.2portant; word-wrap: break-word !important; font-family: 宋体;">％滇麝香草酚蓝溶液 12mL
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
PH 7.4
portant; word-wrap: break-word !important; font-family: 宋体;">制法
portant; word-wrap: break-word !important; line-height: inherit;">1.portant; word-wrap: break-word !important;"> portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">葡萄糖发酵管按上述成分配好后portant; word-wrap: break-word !important; line-height: inherit;">,portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">按portant; word-wrap: break-word !important; line-height: inherit;">0.5portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">％加入葡萄糖portant; word-wrap: break-word !important; line-height: inherit;">,portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">分装于有一个倒置小管的小试管内，portant; word-wrap: break-word !important; line-height: inherit;">121portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">℃高压灭菌portant; word-wrap: break-word !important; line-height: inherit;">15minportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">。
portant; word-wrap: break-word !important; line-height: inherit;">2.portant; word-wrap: break-word !important;"> portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">其他各种糖发酵管可按上述成分配好后，分装每瓶portant; word-wrap: break-word !important; line-height: inherit;">1000 mLportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">，portant; word-wrap: break-word !important; line-height: inherit;">121portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">℃高压灭菌portant; word-wrap: break-word !important; line-height: inherit;">15minportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">。另将各种糖类分别配好portant; word-wrap: break-word !important; line-height: inherit;">10%portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">溶液，同时高压灭菌。将portant; word-wrap: break-word !important; line-height: inherit;">5mLportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">糖溶液加入于portant; word-wrap: break-word !important; line-height: inherit;">100 mLportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋体;">培养基内，以无菌操作分装小试管。
portant; word-wrap: break-word !important; font-family: 宋体;">注：蔗糖不纯，加热后会自行水解者，应采用过滤法除菌。
portant; word-wrap: break-word !important; font-family: 宋体;">试验方法：portant; word-wrap: break-word !important; font-family: 宋体;">从琼脂斜面上挑取小量培养物接种，于36portant; word-wrap: break-word !important; font-family: 宋体;">℃±1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养，一般观察2portant; word-wrap: break-word !important; font-family: 宋体;">～3dportant; word-wrap: break-word !important; font-family: 宋体;">。迟缓反应需观察14~30dportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">二、乳糖胆盐发酵管
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 20g
portant; word-wrap: break-word !important; font-family: 宋体;">猪胆盐（或牛、羊胆盐）5g
portant; word-wrap: break-word !important; font-family: 宋体;">乳糖 10g
0.04%portant; word-wrap: break-word !important; font-family: 宋体;">滇甲酚紫水溶液 25mL
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将蛋白胨，胆盐及乳糖溶于水中，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，加入指示剂，分装每管10 mLportant; word-wrap: break-word !important; font-family: 宋体;">，并放入一个小倒管，115portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">注：双料乳糖胆盐发酵管除蒸馏水外，其他成分加倍。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">三、5portant; word-wrap: break-word !important;">％乳糖发酵管
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 0.2g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 0.5g
portant; word-wrap: break-word !important; font-family: 宋体;">乳糖 5g
2%portant; word-wrap: break-word !important; font-family: 宋体;">溴麝香草酚蓝水溶液 1.2mL
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 100mL
pH 7.4
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">除乳糖以外的各成分：溶解于50mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水内，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">。将乳糖溶解于另外50mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水内，分别灭菌121 portant; word-wrap: break-word !important; font-family: 宋体;">℃ 15minportant; word-wrap: break-word !important; font-family: 宋体;">，将两液混合，以无菌操作分装于灭菌小试管内。
portant; word-wrap: break-word !important; font-family: 宋体;">注：在此培养基内，大部分乳糖迟缓发酵的细菌可于ldportant; word-wrap: break-word !important; font-family: 宋体;">内发酵。
portant; word-wrap: break-word !important; font-size: 18px;">portant; word-wrap: break-word !important; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important; font-family: 宋体;">四、缓冲葡萄糖蛋白胨水（MRportant; word-wrap: break-word !important; font-family: 宋体;">和VPportant; word-wrap: break-word !important; font-family: 宋体;">试验用）
portant; word-wrap: break-word !important; font-family: 宋体;">成分portant; word-wrap: break-word !important; font-family: 宋体;">：
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钾 5g
portant; word-wrap: break-word !important; font-family: 宋体;">多胨 7g
portant; word-wrap: break-word !important; font-family: 宋体;">葡萄糖 5g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">溶化后校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，分装试管，每管1mL portant; word-wrap: break-word !important; font-family: 宋体;">，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">甲基红（MRportant; word-wrap: break-word !important; font-family: 宋体;">）试验：portant; word-wrap: break-word !important; font-family: 宋体;">自琼脂斜面挑取少量培养物接种本培养基中，于36portant; word-wrap: break-word !important; font-family: 宋体;">±1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养2portant; word-wrap: break-word !important; font-family: 宋体;">～5dportant; word-wrap: break-word !important; font-family: 宋体;">，哈夫尼亚菌则应在22portant; word-wrap: break-word !important; font-family: 宋体;">～25portant; word-wrap: break-word !important; font-family: 宋体;">℃培养。滴加甲基红试剂一滴，立即观察结果。鲜红色为阳性，黄色为阴性，甲基红试剂配法：10mgportant; word-wrap: break-word !important; font-family: 宋体;">甲基红溶于30mL95portant; word-wrap: break-word !important; font-family: 宋体;">％乙醇中，然后加入20 mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水。
V-Pportant; word-wrap: break-word !important; font-family: 宋体;">试验：portant; word-wrap: break-word !important; font-family: 宋体;">用琼脂培养物接种本培养基中，于36portant; word-wrap: break-word !important; font-family: 宋体;">±1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养2portant; word-wrap: break-word !important; font-family: 宋体;">～4dportant; word-wrap: break-word !important; font-family: 宋体;">。哈夫尼亚菌则应在22portant; word-wrap: break-word !important; font-family: 宋体;">～25portant; word-wrap: break-word !important; font-family: 宋体;">℃培养。加入6portant; word-wrap: break-word !important; font-family: 宋体;">％α-portant; word-wrap: break-word !important; font-family: 宋体;">萘酚-portant; word-wrap: break-word !important; font-family: 宋体;">乙醇溶液0.5mLportant; word-wrap: break-word !important; font-family: 宋体;">和40portant; word-wrap: break-word !important; font-family: 宋体;">％氢氧化钾溶液0.2mLportant; word-wrap: break-word !important; font-family: 宋体;">充分振摇试管，观察结果阳性反应立刻或于数分钟内出现红色，如为阴性，应放在36portant; word-wrap: break-word !important; font-family: 宋体;">±1portant; word-wrap: break-word !important; font-family: 宋体;">℃下培养4hportant; word-wrap: break-word !important; font-family: 宋体;">再进行观察。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">五、营养明胶
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 5g
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏 3g
portant; word-wrap: break-word !important; font-family: 宋体;">明胶 120g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
pH 6.8~7.0
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">加热溶解，校正至pH7.4portant; word-wrap: break-word !important; font-family: 宋体;">～7.6portant; word-wrap: break-word !important; font-family: 宋体;">，分装小管，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌10minportant; word-wrap: break-word !important; font-family: 宋体;">，取出后迅速冷却，使其凝固，复查最终pHportant; word-wrap: break-word !important; font-family: 宋体;">应为6.8portant; word-wrap: break-word !important; font-family: 宋体;">～7.0portant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">试验方法：portant; word-wrap: break-word !important; font-family: 宋体;">用琼脂培养物穿刺接种，放在22portant; word-wrap: break-word !important; font-family: 宋体;">～25portant; word-wrap: break-word !important; font-family: 宋体;">℃培养，每天观察结果，记录液化时间。或放在36portant; word-wrap: break-word !important; font-family: 宋体;">士1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养，每天取出，放冰箱内30minportant; word-wrap: break-word !important; font-family: 宋体;">后再观察结果。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">六、苯丙氨酸培养基
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">酵母浸膏 3g
DI-portant; word-wrap: break-word !important; font-family: 宋体;">苯丙氨酸（或L-portant; word-wrap: break-word !important; font-family: 宋体;">苯丙氨酸1gportant; word-wrap: break-word !important; font-family: 宋体;">）2g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钠 1g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂 12g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">加热溶解后分装试管，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">，使成斜面。
portant; word-wrap: break-word !important; font-family: 宋体;">试验方法：portant; word-wrap: break-word !important; font-family: 宋体;">自琼脂斜面上挑取大量培养物，移种于苯丙氨酸琼脂，在36portant; word-wrap: break-word !important; font-family: 宋体;">士1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养4hportant; word-wrap: break-word !important; font-family: 宋体;">或18portant; word-wrap: break-word !important; font-family: 宋体;">～24hportant; word-wrap: break-word !important; font-family: 宋体;">，滴加10portant; word-wrap: break-word !important; font-family: 宋体;">％三氯化铁溶液2portant; word-wrap: break-word !important; font-family: 宋体;">～3portant; word-wrap: break-word !important; font-family: 宋体;">滴，自斜面培养物上流下，苯丙氨酸脱氨酶阳性者呈深绿色。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">七、蛋白胨水（靛基质试验用）
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨（或胰蛋白胨）20g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
pH7.4
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">按上述成分配制，分装小试管，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">靛基质试剂
portant; word-wrap: break-word !important; font-family: 宋体;">柯凡克试剂：将5gportant; word-wrap: break-word !important; font-family: 宋体;">对二甲氨基苯甲醛溶解于75mLportant; word-wrap: break-word !important; font-family: 宋体;">戊醇中，然后缓慢加入浓盐酸25mlportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">欧-portant; word-wrap: break-word !important; font-family: 宋体;">波试剂：将1gportant; word-wrap: break-word !important; font-family: 宋体;">对二甲氨基苯甲醛溶解于95mL95%portant; word-wrap: break-word !important; font-family: 宋体;">乙醇内，然后缓慢加入浓盐酸20 mlportant; word-wrap: break-word !important; font-family: 宋体;">。试验方法：挑取小量培养物接种，在36portant; word-wrap: break-word !important; font-family: 宋体;">士1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养1portant; word-wrap: break-word !important; font-family: 宋体;">～2dportant; word-wrap: break-word !important; font-family: 宋体;">，必要时可培养4portant; word-wrap: break-word !important; font-family: 宋体;">～5dportant; word-wrap: break-word !important; font-family: 宋体;">。加入柯凡克试剂约0.5mLportant; word-wrap: break-word !important; font-family: 宋体;">，轻摇试管，阳性者于试剂层呈深红色；或加入欧-portant; word-wrap: break-word !important; font-family: 宋体;">波试剂约0.5 mLportant; word-wrap: break-word !important; font-family: 宋体;">，沿管壁流下，覆盖于培养液表面，阳性者于液面接触处呈玫瑰红色。
portant; word-wrap: break-word !important; font-family: 宋体;">注：蛋白胨中应含有丰富的色氨酸。每批蛋白胨买来后，应先用已知菌种鉴定后方可使用。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">八、硫酸亚铁琼脂（硫化氢试验用）
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏 3g
portant; word-wrap: break-word !important; font-family: 宋体;">酵母浸膏 3g
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋体;">硫酸亚铁 0.2g
portant; word-wrap: break-word !important; font-family: 宋体;">硫代硫酸钠 0.3g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂 12g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000 mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">加热溶解，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，分装试管，115portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">，取出直立候其凝固。
portant; word-wrap: break-word !important; font-family: 宋体;">试验方法：挑取琼脂培养物，沿管壁穿刺，于36portant; word-wrap: break-word !important; font-family: 宋体;">士1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养1portant; word-wrap: break-word !important; font-family: 宋体;">～2dportant; word-wrap: break-word !important; font-family: 宋体;">，观察结果。产硫化氢者使培养基变为黑色。
portant; word-wrap: break-word !important; font-family: 宋体;">注：肠杆菌科细菌测定硫化氢的产生，应采用三糖铁琼脂或本培养基。
portant; word-wrap: break-word !important; font-size: 18px; font-family: 宋体; color: rgb(0, 128, 255);">九、尿素琼脂
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 1g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠5gportant; word-wrap: break-word !important; font-family: 宋体;">葡萄糖1g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸二氢钾 2g
4%portant; word-wrap: break-word !important; font-family: 宋体;">酚红溶液 3 mL
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂 20g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000 mL
20%portant; word-wrap: break-word !important; font-family: 宋体;">尿素溶液 100mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将除尿素和琼脂以外的成分配好，并校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，加入琼脂，加热溶化并分装烧瓶。121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。冷至50portant; word-wrap: break-word !important; font-family: 宋体;">～55portant; word-wrap: break-word !important; font-family: 宋体;">℃，加入经除菌过滤的尿素溶液，尿素的最终浓度为2portant; word-wrap: break-word !important; font-family: 宋体;">％，最终pHportant; word-wrap: break-word !important; font-family: 宋体;">应为7.2portant; word-wrap: break-word !important; font-family: 宋体;">±0.1.portant; word-wrap: break-word !important; font-family: 宋体;">分装于灭菌试管内，放成斜面备用。
portant; word-wrap: break-word !important; font-family: 宋体;">试验方法：portant; word-wrap: break-word !important; font-family: 宋体;">挑取琼脂培养物接种，在36portant; word-wrap: break-word !important; font-family: 宋体;">士1portant; word-wrap: break-word !important; font-family: 宋体;">℃培养24hportant; word-wrap: break-word !important; font-family: 宋体;">，观察结果。尿素酶阳性者由于产碱而使培养基变为红色。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十、氰化钾（KCNportant; word-wrap: break-word !important;">）培养基
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨10g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸二氢钾 0.225g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钠 5.64g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000 mL
0.5%portant; word-wrap: break-word !important; font-family: 宋体;">氰化钾溶液 20 mL
pH 7.6
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将除氰化钾以外的成分配好后分装烧瓶，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。放在冰箱内使其充分冷却。每100 mLportant; word-wrap: break-word !important; font-family: 宋体;">培养基加入0.5portant; word-wrap: break-word !important; font-family: 宋体;">％氰化钾溶液2.0mLportant; word-wrap: break-word !important; font-family: 宋体;">（最后浓度为1portant; word-wrap: break-word !important; font-family: 宋体;">：10000portant; word-wrap: break-word !important; font-family: 宋体;">），分装于12mm*100mmportant; word-wrap: break-word !important; font-family: 宋体;">灭菌试管，每管约4mLportant; word-wrap: break-word !important; font-family: 宋体;">，立刻用灭菌橡皮塞塞紧，放在4portant; word-wrap: break-word !important; font-family: 宋体;">℃冰箱内，至少可保存两个月。同时，将不加氰化钾的培养基作为对照培养基，分装试管备用。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">十一、营养琼脂
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨10g
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏3g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠5g
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂15portant; word-wrap: break-word !important; font-family: 宋体;">～20g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水1000mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将除琼脂以外的各成分溶解于蒸馏水内，加入15%portant; word-wrap: break-word !important; font-family: 宋体;">氢氧化钠溶液约2mLportant; word-wrap: break-word !important; font-family: 宋体;">，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">至7.2~7.4portant; word-wrap: break-word !important; font-family: 宋体;">。加入琼脂，加热煮沸，使琼脂溶化分装烧瓶，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">注：此培养基可供一般细菌培养之用，可倾注平板或制成斜面。如用于菌落计数，琼脂量为1.5%portant; word-wrap: break-word !important; font-family: 宋体;">；如作成平板或斜面，则应为2%portant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">十二、营养肉汤
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏 3g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
pH7.4
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">按上述成分混合，溶解后校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，分装烧瓶，每瓶225 mL portant; word-wrap: break-word !important; font-family: 宋体;">，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十三、缓冲蛋白胨水（BPportant; word-wrap: break-word !important;">）
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钠 9g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸二氢钾 1.5g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水1000mL
pH 7.2
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">按上述成分配好后以大烧瓶装，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。临用时无菌分装每瓶225mLportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">注：本培养基供沙门氏菌前增菌用。
portant; word-wrap: break-word !important; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 18px;">portant; word-wrap: break-word !important; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important; font-family: 宋体;">十四、ECportant; word-wrap: break-word !important; font-family: 宋体;">肉汤
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">胰蛋白胨 20g
3portant; word-wrap: break-word !important; font-family: 宋体;">号胆盐（或混合胆盐）1.5g
portant; word-wrap: break-word !important; font-family: 宋体;">乳糖 5g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钾 4g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸二氢钾 1.5g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将上述成分混合，溶解后，分装有发酵倒管的试管中，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。最终pHportant; word-wrap: break-word !important; font-family: 宋体;">为6.9portant; word-wrap: break-word !important; font-family: 宋体;">±0.2portant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十五、四硫磺酸钠煌绿增菌液（TTBportant; word-wrap: break-word !important;">）
portant; word-wrap: break-word !important; font-family: 宋体;">基础培养基
portant; word-wrap: break-word !important; font-family: 宋体;">多胨或眎胨 5g
portant; word-wrap: break-word !important; font-family: 宋体;">胆盐 1g
portant; word-wrap: break-word !important; font-family: 宋体;">碳酸钙 10g
portant; word-wrap: break-word !important; font-family: 宋体;">硫代硫酸钠 3g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
portant; word-wrap: break-word !important; font-family: 宋体;">碘溶液
portant; word-wrap: break-word !important; font-family: 宋体;">碘 6g
portant; word-wrap: break-word !important; font-family: 宋体;">碘化钾 5g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 20 mL
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将基础培养基的各成分加入蒸馏水中，加热溶解，分装每瓶100mLportant; word-wrap: break-word !important; font-family: 宋体;">。分装时应随时振摇，使其中的碳酸钙混匀，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">备用。临用时每100mLportant; word-wrap: break-word !important; font-family: 宋体;">基础培养基中加入碘溶液2mLportant; word-wrap: break-word !important; font-family: 宋体;">，,0.1%portant; word-wrap: break-word !important; font-family: 宋体;">煌绿溶液1mLportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十六、亚硒酸盐胱氨酸增菌液（SCportant; word-wrap: break-word !important;">）
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 5g
portant; word-wrap: break-word !important; font-family: 宋体;">乳糖 4g
portant; word-wrap: break-word !important; font-family: 宋体;">亚硒酸氢钠 4g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钠 5.5g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸二氢钾 4.5g
L-portant; word-wrap: break-word !important; font-family: 宋体;">胱氨酸 0.01g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
1% L-portant; word-wrap: break-word !important; font-family: 宋体;">胱氨酸-portant; word-wrap: break-word !important; font-family: 宋体;">氢氧化钠溶液的配法portant; word-wrap: break-word !important; font-family: 宋体;">：称取L-portant; word-wrap: break-word !important; font-family: 宋体;">胱氨酸0.1gportant; word-wrap: break-word !important; font-family: 宋体;">或（DL-portant; word-wrap: break-word !important; font-family: 宋体;">胱氨酸0.2gportant; word-wrap: break-word !important; font-family: 宋体;">），加1mol/Lportant; word-wrap: break-word !important; font-family: 宋体;">氢氧化钠1.5mLportant; word-wrap: break-word !important; font-family: 宋体;">，使溶解，再加入蒸馏水8.5mLportant; word-wrap: break-word !important; font-family: 宋体;">即成。
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将除亚硒酸氢钠和L-portant; word-wrap: break-word !important; font-family: 宋体;">胱氨酸以外的各成分溶解于900mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水中，加热煮沸，候冷备用。另将亚硒酸氢钠溶解于100mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水中，加热煮沸，候冷，以无菌操作与上液混合。再加入1% L-portant; word-wrap: break-word !important; font-family: 宋体;">胱氨酸-portant; word-wrap: break-word !important; font-family: 宋体;">氢氧化钠溶液1mLportant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-family: 宋体;">分装于灭菌瓶中．每瓶100mLportant; word-wrap: break-word !important; font-family: 宋体;">，pHportant; word-wrap: break-word !important; font-family: 宋体;">应为7.0portant; word-wrap: break-word !important; font-family: 宋体;">±0.1portant; word-wrap: break-word !important; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十七、亚硫酸铋琼脂（BSportant; word-wrap: break-word !important;">）
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏 5g
portant; word-wrap: break-word !important; font-family: 宋体;">葡萄糖 5g
portant; word-wrap: break-word !important; font-family: 宋体;">硫酸亚铁 0.3g
portant; word-wrap: break-word !important; font-family: 宋体;">磷酸氢二钠 4g
portant; word-wrap: break-word !important; font-family: 宋体;">煌绿 0.025g
portant; word-wrap: break-word !important; font-family: 宋体;">柠檬酸铋铵 2g
portant; word-wrap: break-word !important; font-family: 宋体;">亚硫酸钠 6g
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂 18~20g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000mL
pH 7.5
portant; word-wrap: break-word !important; font-family: 宋体;">制法：
1.portant; word-wrap: break-word !important; font-family: 宋体;">将前面5portant; word-wrap: break-word !important; font-family: 宋体;">种成分溶解于300mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水中。
2portant; word-wrap: break-word !important; font-family: 宋体;">将柠檬酸铋铵和亚硫酸钠另用50mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水溶解。
3portant; word-wrap: break-word !important; font-family: 宋体;">将琼脂于600mLportant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水中煮沸溶解，冷至80portant; word-wrap: break-word !important; font-family: 宋体;">℃。
4portant; word-wrap: break-word !important; font-family: 宋体;">将以上三液合并，补充蒸馏水至1000mLportant; word-wrap: break-word !important; font-family: 宋体;">，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，加0.5%portant; word-wrap: break-word !important; font-family: 宋体;">煌绿水溶液5mLportant; word-wrap: break-word !important; font-family: 宋体;">，摇匀，冷至50portant; word-wrap: break-word !important; font-family: 宋体;">～55portant; word-wrap: break-word !important; font-family: 宋体;">℃，倾注平皿。
portant; word-wrap: break-word !important; font-family: 宋体;">注：此培养基不需高压灭菌。制备过程不宜过分加热，以免降低其选择性。应在临用前一天制备，贮存于室温暗处，超过48hportant; word-wrap: break-word !important; font-family: 宋体;">不宜使用。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十八、三糖铁琼脂（TSIportant; word-wrap: break-word !important;">）
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨 20g
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏 5g
portant; word-wrap: break-word !important; font-family: 宋体;">乳糖 10g
portant; word-wrap: break-word !important; font-family: 宋体;">蔗糖 10g
portant; word-wrap: break-word !important; font-family: 宋体;">葡萄糖 1g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">硫酸亚铁铵 0.2g
portant; word-wrap: break-word !important; font-family: 宋体;">硫代硫酸钠 0.2g
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂 12g
portant; word-wrap: break-word !important; font-family: 宋体;">酚红 0.025g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000 mL
pH 7.4portant; word-wrap: break-word !important; line-height: inherit;">
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将除琼脂和酚红以外的各成分溶解于蒸馏水中，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，加入琼脂，加热煮沸，以溶化琼脂。加入0.2%portant; word-wrap: break-word !important; font-family: 宋体;">酚红水溶液12.5 mLportant; word-wrap: break-word !important; font-family: 宋体;">，摇匀，分装试管。装量宜多些，以便得到较高的底层。121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">，放置高层斜面备用。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">十九、三糖铁琼脂换用方法
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨15g
portant; word-wrap: break-word !important; font-family: 宋体;">眎胨5g
portant; word-wrap: break-word !important; font-family: 宋体;">牛肉膏 3g
portant; word-wrap: break-word !important; font-family: 宋体;">酵母膏 3g
portant; word-wrap: break-word !important; font-family: 宋体;">乳糖 10g
portant; word-wrap: break-word !important; font-family: 宋体;">蔗糖 10g
portant; word-wrap: break-word !important; font-family: 宋体;">葡萄糖 1g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 5g
portant; word-wrap: break-word !important; font-family: 宋体;">硫酸亚铁 0.2g
portant; word-wrap: break-word !important; font-family: 宋体;">硫代硫酸钠 0.3g
portant; word-wrap: break-word !important; font-family: 宋体;">琼脂 12g
portant; word-wrap: break-word !important; font-family: 宋体;">酚红 0.025g
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水 1000 mL
pH7.4
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">将除琼脂和酚红以外的各成分溶解于蒸馏水中，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，加入琼脂，加热煮沸，以溶化琼脂。加入0.2%portant; word-wrap: break-word !important; font-family: 宋体;">酚红水溶液12.5 mLportant; word-wrap: break-word !important; font-family: 宋体;">，摇匀，分装试管，装量宜多些，以便得到较高的底层。121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">，放置高层斜面备用。
portant; word-wrap: break-word !important; font-family: 宋体; font-size: 18px; color: rgb(0, 128, 255);">二十、嗜盐性试验培养基
portant; word-wrap: break-word !important; font-family: 宋体;">成分：
portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨2g
portant; word-wrap: break-word !important; font-family: 宋体;">氯化钠 portant; word-wrap: break-word !important; font-family: 宋体;">按不同量加
portant; word-wrap: break-word !important; font-family: 宋体;">蒸馏水100 mL
pH7.7
portant; word-wrap: break-word !important; font-family: 宋体;">制法：portant; word-wrap: break-word !important; font-family: 宋体;">配制2%portant; word-wrap: break-word !important; font-family: 宋体;">蛋白胨水，校正pHportant; word-wrap: break-word !important; font-family: 宋体;">，共配制5portant; word-wrap: break-word !important; font-family: 宋体;">瓶，每瓶100mLportant; word-wrap: break-word !important; font-family: 宋体;">。每瓶分别加入不同量的氯化钠：（1portant; word-wrap: break-word !important; font-family: 宋体;">）不加；（2portant; word-wrap: break-word !important; font-family: 宋体;">）3gportant; word-wrap: break-word !important; font-family: 宋体;">；（3portant; word-wrap: break-word !important; font-family: 宋体;">）7gportant; word-wrap: break-word !important; font-family: 宋体;">；（4portant; word-wrap: break-word !important; font-family: 宋体;">）9gportant; word-wrap: break-word !important; font-family: 宋体;">；（5portant; word-wrap: break-word !important; font-family: 宋体;">）11gportant; word-wrap: break-word !important; font-family: 宋体;">。待溶解后分装试管，121portant; word-wrap: break-word !important; font-family: 宋体;">℃高压灭菌15minportant; word-wrap: break-word !important; font-family: 宋体;">。
