portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">设定移液体积：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">从大量程调节至小量程为正常调节方法，逆时针旋转刻度即可；portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">从小量程调节至大量程时，应先调至超过设定体积刻度，再回调至设定体积，这样可以保证移液器的精确度。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">2.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">装配移液枪头：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">将移液枪垂直插入吸头，左右旋转半圈，上紧即可。portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">（注意：用移液器撞击吸头的方法是非常不可取的，长期这样操作回导致移液器的零件因撞击而松散，严重会导致调节刻度的旋钮卡住。）
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">吸液及放液：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">垂直吸液portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; text-decoration: underline;">portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">吸头尖端浸入液面portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3mmportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">以下，吸液前枪头先在液体中预润洗portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">2portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">～portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">次确保移液的精度和准度portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">（因为吸头内壁会残留一层“液膜”，造成排液量偏小而产生误差）；portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">慢吸慢放portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">，以防突然松开溶液吸入过快而冲入取液器内腐蚀柱塞而造成漏气；portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">放液时如果量很小则应吸头尖端可靠容器内壁。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">（使用时要检查是否有漏液现象。方法是吸取液体后悬空垂直放置几秒中，看看液面是否下降。如果漏液，则检查吸液嘴是否匹配和弹簧活塞是否正常。）
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">4. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">浓度和粘度大的液体：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">会产生误差，为消除其误差的补偿量，可由试验确定，补偿量可用调节旋钮改变读数窗的读数来进行设定。（可采用portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">反向移液技术portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移取高粘度液体）
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">5portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">吸有液体的移液枪不应平放：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">枪头内的液体很容易污染枪内部而可能导致枪的弹簧生锈。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">6portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液枪在每次实验后应将刻度调至最大portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">让弹簧回复原型以延长移液枪的使用寿命。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">7portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液枪校正：
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">可用分析天平称量所取纯水的重量并进行计算的方法，来校正取液器，portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1mLportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">蒸馏水portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">20portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">℃时重portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">0.9982gportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">8portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">移液器严禁吸取有强挥发性、强腐蚀性的液体（如，浓酸、浓碱、有机物等portant; word-wrap: break-word !important;">）portant; word-wrap: break-word !important;">。
portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">9portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">严禁使用移液器吹打混匀液体。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">10portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">不要用大量程的移液器移取小体积的液体，以免影响准确度；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">同时，如果需要移取量程范围以外较大量的液体，请使用移液管进行操作。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">如液体不小心进入活塞室应及时清除污染物；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">定期清洁移液枪外壁，可以用portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">95%portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">酒精或portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">60%portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">的异丙醇，再用蒸馏水擦拭，自然晾干。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器使用注意事项
portant; word-wrap: break-word !important; font-size: 14px; font-family: 微软雅黑, sans-serif; color: rgb(85, 85, 85);">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(1)吸取液体时一定要缓慢平稳地松开拇指，绝不允许突然松开，以防将溶液吸入过快而冲入取液器内腐蚀柱塞而造成漏气。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(2)为获得较高的精度，吸头需预先吸取一次样品溶液，然后再正式移液，因为吸取血清蛋白质溶液或有机溶剂时，吸头内壁会残留一层“液膜”，造成排液量偏小而产生误差。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(3)浓度和粘度大的液体，会产生误差，为消除其误差的补偿量，可由试验确定，补偿量可用调节旋钮改变读数窗的读数来进行设定。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(4)可用分析天平称量所取纯水的重量并进行计算的方法，来校正取液器，1mL蒸馏水20℃时重0.9982g。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(5)portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液器portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">反复撞击吸头来上紧的方法是非常不可取的，长期操作会使内部零件松散而损坏移液器。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(6)移液器未装吸头时，切莫移液。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(7)在设置量程时，请注意？旋转到所需量程？数字清清楚楚在显示窗中？所设量程在移液器量程范围内不要将按钮旋出量程，否则会卡住机械装置，损坏了移液器。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(8)移液器严禁吸取有强挥发性、强腐蚀性的液体（如，浓酸、浓碱、有机物等）。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(9)严禁使用移液器吹打混匀液体。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(10)不要用大量程的移液器移取小体积的液体，以免影响准确度。同时，如果需要移取量程范围以外较大量的液体，请使用portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液管portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">进行操作。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器容量允许误差和测量重复性
| portant; word-wrap: break-word !important;">标称容量/μL | portant; word-wrap: break-word !important;">校准点portant; word-wrap: break-word !important;">/μL | portant; word-wrap: break-word !important;">容量允许误差 portant; word-wrap: break-word !important;">±(%) | portant; word-wrap: break-word !important;">测量重复性 portant; word-wrap: break-word !important;">≤(%) |
| portant; word-wrap: break-word !important;">20 | portant; word-wrap: break-word !important;">2 | portant; word-wrap: break-word !important;">12.0 | portant; word-wrap: break-word !important;">6.0 |
| portant; word-wrap: break-word !important;">10 | portant; word-wrap: break-word !important;">8.0 | portant; word-wrap: break-word !important;">4.0 | |
| portant; word-wrap: break-word !important;">20 | portant; word-wrap: break-word !important;">4.0 | portant; word-wrap: break-word !important;">2.0 | |
| portant; word-wrap: break-word !important;">50 | portant; word-wrap: break-word !important;">5 | portant; word-wrap: break-word !important;">8.0 | portant; word-wrap: break-word !important;">4.0 |
| portant; word-wrap: break-word !important;">25 | portant; word-wrap: break-word !important;">4.0 | portant; word-wrap: break-word !important;">2.0 | |
| portant; word-wrap: break-word !important;">50 | portant; word-wrap: break-word !important;">3.0 | portant; word-wrap: break-word !important;">1.5 | |
| portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">10 | portant; word-wrap: break-word !important;">8.0 | portant; word-wrap: break-word !important;">4.0 |
| portant; word-wrap: break-word !important;">50 | portant; word-wrap: break-word !important;">3.0 | portant; word-wrap: break-word !important;">1.5 | |
| portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">2.0 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">200 | portant; word-wrap: break-word !important;">20 | portant; word-wrap: break-word !important;">4.0 | portant; word-wrap: break-word !important;">2.0 |
| portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">2.0 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">200 | portant; word-wrap: break-word !important;">1.5 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">1000 | portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">2.0 | portant; word-wrap: break-word !important;">1.0 |
| portant; word-wrap: break-word !important;">500 | portant; word-wrap: break-word !important;">1.0 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">1000 | portant; word-wrap: break-word !important;">1.0 | portant; word-wrap: break-word !important;">0.5 | |
| portant; word-wrap: break-word !important;">5000 | portant; word-wrap: break-word !important;">500 | portant; word-wrap: break-word !important;">1.0 | portant; word-wrap: break-word !important;">0.5 |
| portant; word-wrap: break-word !important;">2500 | portant; word-wrap: break-word !important;">0.5 | portant; word-wrap: break-word !important;">0.2 | |
| portant; word-wrap: break-word !important;">5000 | portant; word-wrap: break-word !important;">0.6 | portant; word-wrap: break-word !important;">0.2 |
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器快速校准的方法
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">使用设备及物品：电子天平（测量范围200g；分度值0.1mg）；烧杯（50mL）1个；烧杯（100mL）1个；定性滤纸若干小块；超纯水80mL；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">1.打开电子天平，空载调零；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">2.放入小烧杯待天平显示稳定后，按下归零键归零。给移液器上安装相应的吸液嘴，对移液器的枪头进行预洗；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">3.将移液器的容量调至被检点。被检点一般选择最小，中间和最大容量值。可以是某一指定的容量点；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">4.使用移液器移取被检容量的超纯水至天平的小烧杯内；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">5.待天平显示稳定后，马上读取并记录此时电子天平显示出的数值。记录完毕将电子天平复零；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">6.重复5次执行3-5条。记录完毕，将移液器的容量调至下一被检点继续。被检点遵循从小到大的原则进行调整；
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的评价标准
portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">
portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">表一.移液器容量允许误差和测量重复性
portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold;">
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的修正方法
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">1.将移液器调整至最小量程，按照校准方法得到当前的实际容量值，根据与标示容量值的差值，通过移液器上的调准旋钮进行调整；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">2.当调整后测得的实际容量值与标示容量值符合表1要求时，将移液器容量调整至中间量程继续测量。中间量程符合要求时，再调整至最大量程继续测量。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">3.如果中间量程或者最大量程有一个不符合表1要求时，则需重新调整至最小量程继续修正。直到3个量程的值都符合表1要求为止。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">4.修正完毕的移液器，需重新进行校准。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">5.最小量程低于10μL的，不进行修正。如果最大量程和中间量程符合校准要求的，继续使用，否则返厂维修。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的日常维护保养
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">1.如长时间不使用，要把移液枪的量程调至最大值的刻度，使弹簧处于松弛状态以保护弹簧；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">2.定期清洁移液器外壁，可以用95%酒精或60%的异丙醇，再用蒸馏水擦拭，自然晾干；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">3.使用时要检查是否有漏液现象。方法是吸取液体后悬空垂直放置几秒中，看看液面是否下降。如果漏液，则检查吸液嘴是否匹配和弹簧活塞是否正常；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">4.当移液器吸嘴内有液体时，严禁将移液器水平或倒置放置，以防液体流入活塞室腐蚀移液器活塞；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">5.使用正确的方法安装吸头，切记用力不能过猛，更不能采取剁吸头的方法来进行安装；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">小结portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">需要提醒注意的是：移液器如果需要消毒可以用紫外照，或者用75%酒精擦拭，且不可近火呦。
