盐池滩羊免费吃
世界食品网

移液器的使用方法、注意事项以及日常保养维护方法汇总!

   2018-05-22 食品实验室服务公众号243
核心提示:移液器的使用方法portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: bre
 移液器的使用方法

 

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">设定移液体积:portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">从大量程调节至小量程为正常调节方法,逆时针旋转刻度即可;portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">从小量程调节至大量程时,应先调至超过设定体积刻度,再回调至设定体积,这样可以保证移液器的精确度。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">2.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">装配移液枪头:portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">将移液枪垂直插入吸头,左右旋转半圈,上紧即可。portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(注意:用移液器撞击吸头的方法是非常不可取的,长期这样操作回导致移液器的零件因撞击而松散,严重会导致调节刻度的旋钮卡住。)

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">吸液及放液:portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">垂直吸液portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">:portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; text-decoration: underline;">portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">吸头尖端浸入液面portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3mmportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">以下,吸液前枪头先在液体中预润洗portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">2portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">~portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">次确保移液的精度和准度portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(因为吸头内壁会残留一层“液膜”,造成排液量偏小而产生误差);portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">慢吸慢放portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">,以防突然松开溶液吸入过快而冲入取液器内腐蚀柱塞而造成漏气;portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">放液时如果量很小则应吸头尖端可靠容器内壁。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(使用时要检查是否有漏液现象。方法是吸取液体后悬空垂直放置几秒中,看看液面是否下降。如果漏液,则检查吸液嘴是否匹配和弹簧活塞是否正常。)

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">4. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">浓度和粘度大的液体:portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">会产生误差,为消除其误差的补偿量,可由试验确定,补偿量可用调节旋钮改变读数窗的读数来进行设定。(可采用portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">反向移液技术portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移取高粘度液体)

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">5portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">吸有液体的移液枪不应平放:portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">枪头内的液体很容易污染枪内部而可能导致枪的弹簧生锈。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">6portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液枪在每次实验后应将刻度调至最大portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">让弹簧回复原型以延长移液枪的使用寿命。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">7portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液枪校正:

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">可用分析天平称量所取纯水的重量并进行计算的方法,来校正取液器,portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1mLportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">蒸馏水portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">20portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">℃时重portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">0.9982gportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">8portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">移液器严禁吸取有强挥发性、强腐蚀性的液体(如,浓酸、浓碱、有机物等portant; word-wrap: break-word !important;">)portant; word-wrap: break-word !important;">。

portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">9portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">严禁使用移液器吹打混匀液体。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">10portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">不要用大量程的移液器移取小体积的液体,以免影响准确度;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">同时,如果需要移取量程范围以外较大量的液体,请使用移液管进行操作。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋体;">如液体不小心进入活塞室应及时清除污染物;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">定期清洁移液枪外壁,可以用portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">95%portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">酒精或portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">60%portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">的异丙醇,再用蒸馏水擦拭,自然晾干。

 

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器使用注意事项

portant; word-wrap: break-word !important; font-size: 14px; font-family: 微软雅黑, sans-serif; color: rgb(85, 85, 85);"> 

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(1)吸取液体时一定要缓慢平稳地松开拇指,绝不允许突然松开,以防将溶液吸入过快而冲入取液器内腐蚀柱塞而造成漏气。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(2)为获得较高的精度,吸头需预先吸取一次样品溶液,然后再正式移液,因为吸取血清蛋白质溶液或有机溶剂时,吸头内壁会残留一层“液膜”,造成排液量偏小而产生误差。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(3)浓度和粘度大的液体,会产生误差,为消除其误差的补偿量,可由试验确定,补偿量可用调节旋钮改变读数窗的读数来进行设定。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(4)可用分析天平称量所取纯水的重量并进行计算的方法,来校正取液器,1mL蒸馏水20℃时重0.9982g。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(5)portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液器portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">反复撞击吸头来上紧的方法是非常不可取的,长期操作会使内部零件松散而损坏移液器。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(6)移液器未装吸头时,切莫移液。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(7)在设置量程时,请注意?旋转到所需量程?数字清清楚楚在显示窗中?所设量程在移液器量程范围内不要将按钮旋出量程,否则会卡住机械装置,损坏了移液器。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(8)移液器严禁吸取有强挥发性、强腐蚀性的液体(如,浓酸、浓碱、有机物等)。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(9)严禁使用移液器吹打混匀液体。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">(10)不要用大量程的移液器移取小体积的液体,以免影响准确度。同时,如果需要移取量程范围以外较大量的液体,请使用portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">移液管portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">进行操作。

 

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器容量允许误差和测量重复性

 

portant; word-wrap: break-word !important;">

portant; word-wrap: break-word !important;">标称容量/μL

portant; word-wrap: break-word !important;">校准点portant; word-wrap: break-word !important;">/μL

portant; word-wrap: break-word !important;">容量允许误差

portant; word-wrap: break-word !important;">±(%)

portant; word-wrap: break-word !important;">测量重复性

portant; word-wrap: break-word !important;">≤(%)

portant; word-wrap: break-word !important;">20

portant; word-wrap: break-word !important;">2

portant; word-wrap: break-word !important;">12.0

portant; word-wrap: break-word !important;">6.0

portant; word-wrap: break-word !important;">10

portant; word-wrap: break-word !important;">8.0

portant; word-wrap: break-word !important;">4.0

portant; word-wrap: break-word !important;">20

portant; word-wrap: break-word !important;">4.0

portant; word-wrap: break-word !important;">2.0

portant; word-wrap: break-word !important;">50

portant; word-wrap: break-word !important;">5

portant; word-wrap: break-word !important;">8.0

portant; word-wrap: break-word !important;">4.0

portant; word-wrap: break-word !important;">25

portant; word-wrap: break-word !important;">4.0

portant; word-wrap: break-word !important;">2.0

portant; word-wrap: break-word !important;">50

portant; word-wrap: break-word !important;">3.0

portant; word-wrap: break-word !important;">1.5

portant; word-wrap: break-word !important;">100

portant; word-wrap: break-word !important;">10

portant; word-wrap: break-word !important;">8.0

portant; word-wrap: break-word !important;">4.0

portant; word-wrap: break-word !important;">50

portant; word-wrap: break-word !important;">3.0

portant; word-wrap: break-word !important;">1.5

portant; word-wrap: break-word !important;">100

portant; word-wrap: break-word !important;">2.0

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">200

portant; word-wrap: break-word !important;">20

portant; word-wrap: break-word !important;">4.0

portant; word-wrap: break-word !important;">2.0

portant; word-wrap: break-word !important;">100

portant; word-wrap: break-word !important;">2.0

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">200

portant; word-wrap: break-word !important;">1.5

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">1000

portant; word-wrap: break-word !important;">100

portant; word-wrap: break-word !important;">2.0

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">500

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">1000

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">0.5

portant; word-wrap: break-word !important;">5000

portant; word-wrap: break-word !important;">500

portant; word-wrap: break-word !important;">1.0

portant; word-wrap: break-word !important;">0.5

portant; word-wrap: break-word !important;">2500

portant; word-wrap: break-word !important;">0.5

portant; word-wrap: break-word !important;">0.2

portant; word-wrap: break-word !important;">5000

portant; word-wrap: break-word !important;">0.6

portant; word-wrap: break-word !important;">0.2

 

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器快速校准的方法

 

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">使用设备及物品:电子天平(测量范围200g;分度值0.1mg);烧杯(50mL)1个;烧杯(100mL)1个;定性滤纸若干小块;超纯水80mL;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">1.打开电子天平,空载调零;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">2.放入小烧杯待天平显示稳定后,按下归零键归零。给移液器上安装相应的吸液嘴,对移液器的枪头进行预洗;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">3.将移液器的容量调至被检点。被检点一般选择最小,中间和最大容量值。可以是某一指定的容量点;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">4.使用移液器移取被检容量的超纯水至天平的小烧杯内;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">5.待天平显示稳定后,马上读取并记录此时电子天平显示出的数值。记录完毕将电子天平复零;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">6.重复5次执行3-5条。记录完毕,将移液器的容量调至下一被检点继续。被检点遵循从小到大的原则进行调整;

 

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的评价标准

portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">

portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">表一.移液器容量允许误差和测量重复性

portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold;">

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的修正方法

 

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">1.将移液器调整至最小量程,按照校准方法得到当前的实际容量值,根据与标示容量值的差值,通过移液器上的调准旋钮进行调整;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">2.当调整后测得的实际容量值与标示容量值符合表1要求时,将移液器容量调整至中间量程继续测量。中间量程符合要求时,再调整至最大量程继续测量。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">3.如果中间量程或者最大量程有一个不符合表1要求时,则需重新调整至最小量程继续修正。直到3个量程的值都符合表1要求为止。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">4.修正完毕的移液器,需重新进行校准。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">5.最小量程低于10μL的,不进行修正。如果最大量程和中间量程符合校准要求的,继续使用,否则返厂维修。

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的日常维护保养

 

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">1.如长时间不使用,要把移液枪的量程调至最大值的刻度,使弹簧处于松弛状态以保护弹簧;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">2.定期清洁移液器外壁,可以用95%酒精或60%的异丙醇,再用蒸馏水擦拭,自然晾干;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">3.使用时要检查是否有漏液现象。方法是吸取液体后悬空垂直放置几秒中,看看液面是否下降。如果漏液,则检查吸液嘴是否匹配和弹簧活塞是否正常;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">4.当移液器吸嘴内有液体时,严禁将移液器水平或倒置放置,以防液体流入活塞室腐蚀移液器活塞;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">5.使用正确的方法安装吸头,切记用力不能过猛,更不能采取剁吸头的方法来进行安装;

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">

portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">小结portant; word-wrap: break-word !important; font-family: 宋体; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">

portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋体;">需要提醒注意的是:移液器如果需要消毒可以用紫外照,或者用75%酒精擦拭,且不可近火呦。



 
反对 0举报 0 收藏 0 打赏 0
 
更多>同类商务指南
推荐图文
推荐商务指南
点击排行
 
盐池滩羊