蛋白名称：ICAM-1蛋白, ICAM-1 protein
蛋白构建：A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/ml, 100 μl/well), approximately >50% cells will adhere specifically .
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 28
蛋白分子量：The recombinant mouse ICAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 706 amino acids and has a predicted molecular mass of 78.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1/Fc monomer is approximately 120-130 kDa due to glycosylation.
蛋白NP号：NP_034623.1
蛋白氨基酸序列：Met1-Asn485
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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