美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到质量，并保证产品之间一致性，方便研究客户使用。
以下为Advanced BioMatrix3D Matrices 产品竞争优势：
1. 提供高纯度和成分确定的胞外基质；
2. 超过1000余篇文献引用PureCol产品，品质非常均一；
3. 在3D培养基领域可提供全面的产品线；
4. 可提供特异性刚性硅基板的公司（CytoSoft）;
5. 可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；
6. 如果客户接触3D胶原凝胶，Advanced BioMatrix还是的预制胶原蛋白(PureCol EZ Gel)供应商；
以下产品为Advanced BioMatrix畅销品：
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5010-50ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Selec Array kit Ultra-36 #5170-1EA
7. CytoSoft（刚性可变的基底，Advanced BioMatrix添加产品5190-7EA）
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG
Advanced BioMatrix畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。
许多科研人员正从Matrigel产品使用习惯转换过来，下面简单比较下Advanced BioMatrix公司的PureCol和Matrigel：
| Matrigel | PureCol |
| 浓度不均一 | 均一且浓度精确 |
| 鼠，来源 | 牛，非来源 |
| 未知组分 | 已知且明确的组分 |
| 需在冰上分装操作 | 简单易用 |
客户可从上表对比Matrigel和VitroCol （人I型胶原蛋白），以人源简单代替牛源。
PureCol® EZ Gel--3D胶原凝胶让实验更简单
含牛源I型胶原蛋白（5 mg/ml）的DMEM/F-12 培养基, 并添加谷氨酰胺，中性pH。
商品说明：PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
PureCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.
Purecol胶原蛋白被称为标准纯度的胶原蛋白（>99.9%胶原蛋白含量），其功能可媲美大多数的原生胶原蛋白。Purecol提取于来自美国封闭式牛群的牛皮，生物生产过程符合严格的质量标准，已被证明是质量超凡的。Purecol胶原蛋白是由约97%的I型胶原蛋白，其余的为III型胶原蛋白组成的，它具有很高的单体含量可以用于测试凝胶渗透色谱。本产品的浓度约为3mg/ml，每个批次的具体浓度会写于产品的COA上随货发出。PureCol是溶于0.01N盐酸的端胶原蛋白，因此它的PH值约为2。PureCol胶原蛋白是理想的蛋白质涂层，可用于准备培养的细胞或者固体凝胶。PureCol胶原蛋白的包装便于储存和使用，经过灭菌的溶液随时可以使用。
| 产品信息 | PureCol® 胶原蛋白溶液 货号 # BM-5005-100ML |
| 灭菌方法 | 过滤 |
| 形态 | 溶液 |
| 包装尺寸 | 100 ml |
| 储存温度 | 2-10 °C |
| 有效期 | 详见产品包装与随货发出的COA |
| 浓度（缩二脲蛋白测定） | 2.9-3.2 mg/ml |
| 纯度（胶原蛋白浓度） | > 99.9% |
| pH | pH约为2, 0.01 N HCl |
| 凝胶时间（凝胶时间测定法） | < 40 分钟 |
| 原纤维形成（纤维形成） | >0.5 吸光度单位 |
| 聚丙烯酰胺凝胶电泳（SDS–PAGE） | > 85%含胶原蛋白在α，β，和γ带，< 15%含比α带快的胶原蛋白 |
| 无菌的 | 没有细菌生长 |
| 内毒素 (LAL) | < 1.0 EU/ml |
| 渗透压 | ~ 20 mOsm |
| 电导率 | ~ 2300 µs/cm |
| 细胞附着实验 | 通过 |
| 来源 | 牛皮–胃蛋白酶提取 |
使用方法：
Coating Procedure
Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
Remove required quantity of collagen from the bottle and dispense into a dilution vessel. Dilute PureCol® in water to ~50 to 100 µg/ml (~1:30). A 0.01 M HCl solution may also be used. Swirl contents gently until material is completely mixed. Add appropriate amount of diluted PureCol® material to the culture surface ensuring that the entire surface is coated. Incubate at room temperature or 37°C, covered, for 1-2 hours. After incubation, aspirate any remaining material. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.3-D Gel Preparation Procedure
Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper). Adjust final volume to a total of 10 parts with sterile water. To prevent gelation, maintain temperature of mixture at 2–10°C. To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.Cell Attachment Bioassay
To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
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