| 甲基纤维素培养基—人 |
| 甲基纤维素培养基—人 | ||||||
| 无血清培养基 | 04236 | MethoCult SF H4236, 80 mL | 甲基纤维素培养基MethoCult SFBIT H4236, 80mL | 集落分析基础培养基 | 80 mL | 3,690 |
| 04246 | Special MethoCult SF H4236, 24 x 3mL | 甲基纤维素培养基MethoCult SFBIT H4236, 24x2.4mL | 24 vials | 3,690 | ||
| 04436 | MethoCult SF H4436, 100 mL | 甲基纤维素培养基MethoCult H4436, 100 mL | 支持红系、粒系、多系细胞集落 | 100 mL | 10,682 | |
| 04446 | Special MethoCult SF H4436, 24 x 3mL | 甲基纤维素培养基MethoCult H4436, 24 x 3 mL | 24 vials | 10,682 | ||
| 04536 | MethoCult SF H4536, 100 mL | 甲基纤维素培养基MethoCult H4536, 100 mL | 支持粒系集落，不支持红系集落 | 100 mL | 9,450 | |
| 04546 | Special MethoCult SF H4536, 24 x 3mL | 订制甲基纤维素培养基SF H4536, 24 x 3mL | 24 vials | 9,450 | ||
| 完全培养基 | 04034 | MethoCult H4034 Optimum, 100 mL | 甲基纤维素培养基MethoCult GF+ H4034, 100 mL | 支持红系、粒系、多系细胞集落；用于脐带血的集落更清晰 | 100 mL | 7,450 |
| 04035 | MethoCult Optimum w/o EPO, 100 mL | 甲基纤维素培养基MethoCult H4035, 100mL | 支持粒系集落 | 100 mL | 6,450 | |
| 04044 | MethoCult H4034 Optimum, 24x3mL/tube | 甲基纤维素培养基MethoCult H4034, 24 x 3mL | 24 x 3 mL | 8,425 | ||
| 04045 | MethoCult H4035 Optimum w/o EPO, 24x3mL | 甲基纤维素培养基MethoCult H4035, 24 x 3mL | 24 vials | 7,700 | ||
| 04064 | MethoCult GF H4034 Starter Kit | 甲基纤维素培养基MethoCult H4034 Starter Kit | 1 Kit | 15,525 | ||
| 04434 | MethoCult H4434 Classic, 100 mL | 甲基纤维素培养基MethoCult H4434 Classic,100 mL | 支持红系、粒系、多系细胞集落 | 100 mL | 7,452 | |
| 04435 | MethoCult H4435 Enriched, 100 mL | 甲基纤维素培养基MethoCult H4435, 100 mL | 作LTC-IC后集落分析培养基 | 100 mL | 8,736 | |
| 04444 | MethoCult H4434 Classic, 24x3 mL/tube | 甲基纤维素培养基MethoCult H4434, 24 x 3mL | 24 x 3 mL | 8,257 | ||
| 04445 | MethoCult H4435 Enriched, 24 x 3 mL/tube | 甲基纤维素培养基MethoCult H4435, 24 x 3mL | 24 vials | 12,838 | ||
| 04464 | MethoCult GF H4434 Starter Kit, kit | 订制甲基纤维素培养基 H4434, Starter Kit | 1 Kit | 15,215 | ||
| 不含EPO的完全培养基 | 04534 | MethoCult H4534 Classic w/out EPO,100mL | 甲基纤维素培养基MethoCult H4534 Classic,100mL | 支持粒系集落，不支持红系集落 | 100 mL | 6,600 |
| 04535 | MethoCult H4535 Enriched w/o EPO, 100mL | 甲基纤维素培养基MethoCult H4535 Enriched, 100mL | 作LTC-IC后集落分析培养基 | 100 mL | 8,100 | |
| 04544 | MethoCult Classic w/o EPO, 24 x 3 mL | 甲基纤维素培养基MethoCult GF H4534, 24 x 3mL | 24 x 3 vials | 7,700 | ||
| 04545 | MethoCult Enriched w/o EPO, 24 x 3mL | 甲基纤维素培养基MethoCult GF+ H4535,24x3mL | 24 vials | 11,500 | ||
| 04564 | MethoCult GF H4534 Starter Kit, Kit | 甲基纤维素培养基H4534, Starter Kit | 1 Kit | 15,050 | ||
| 特殊的培养基 | 04001 | MethoCult H4001, 100 mL | 甲基纤维素培养基MethoCult H4001, 100 ml | 检测药物对CFU-GM集落的敏感性，可测粒系，巨核系 | 100 mL | 5,800 |
| 04230 | MethoCult H4230, 80 mL | 甲基纤维素培养基MethoCult H4230, 80 mL | 集落分析基础培养基，不含细胞因子 | 80 mL | 3,450 | |
| 04240 | Special MethoCult H4230, 24 x 2.4mL | 订制甲基纤维素培养基 H4230, 24 x 2.4mL | 24 vials | 3,749 | ||
| 04330 | MethoCult H4330, 90 mL | 甲基纤维素培养基MethoCult H4330, 90 mL | 集落分析基础培养基，含EPO | 90 mL | 6,738 | |
| 04340 | Special MethoCult H4330, 24 x 2.7mL | 甲基纤维素培养基MethoCult H4330, 24 x 2.7mL | 24 vials | 6,738 | ||
| 集落快速分析培养基 | 04437 | MethoCult Express, 100mL | 甲基纤维素培养基MethoCult Express, 100mL | 100 mL | 10,290 | |
| 04447 | MethoCult Express Racks, 3mL/Vial | 甲基纤维素培养基MethoCult Express, 24 x 3mL | 24 Vials | 13,377 | ||
| 基础培养基 | 04100 | MethoCult H4100, 40 mL | 甲基纤维素培养基MethoCult H4100, 40 mL | 含2.6%甲基纤维素的IMDM | 40 mL | 1,225 |
| 含PHA-LCM的完全培养基 | 04433 | MethoCult H4433, 100mL | 甲基纤维素培养基MethoCult H4433, 100mL | 支持红系、粒系、多系细胞集落 | 100 mL | 9,090 |
| 04443 | Special MethoCult H4433, 24 x 3mL | 甲基纤维素培养基MethoCult H4433, 100mL | 24 vials | 9,200 | ||
| 04463 | Special MethoCult H4433, Starter Kit | 订制甲基纤维素培养基H4433, Starter Kit | 1 Custom | 15,705 | ||
| 含PHA-LCM的完全培养（不含EPO） | 04533 | MethoCult H4533, 100mL | 甲基纤维素培养基MethoCult H4533,100mL | 支持粒系集落，不支持红系集落 | 100 mL | 7,375 |
| 04563 | Special MethoCult H4533, Starter Kit | 订制甲基纤维素培养基H4533, Starter Kit | 1 Custom | 15,050 | ||
| 含Agar－LCM的完全培养基（不含EPO） | 04531 | MethoCult H4531, 100mL | 甲基纤维素培养基MethoCult H4531,100mL | 支持粒系集落 | 100 mL | 7,179 |
| 04541 | Special MethoCult H4531, 24 x 3mL | 订制甲基纤维素培养基MethoCult H4531,24x3mL | 24 vials | 12,500 | ||
| 04561 | Special MethoCult H4531, Starter Kit | 订制甲基纤维素培养基H4531, Starter Kit | 1 Custom | 15,050 | ||
| 含Agar－LCM的完全培养基 | 04431 | MethoCult H4431, 100mL | 甲基纤维素培养基MethoCult H4431, 100mL | 支持红系、粒系、多系细胞集落 | 100 mL | 8,012 |
| 04441 | MethoCult H4431, 24 x 3 mL | 甲基纤维素培养基MethoCult H4431, 24 x 3mL | 24 vials | 11,564 | ||
| 04461 | Special MethoCult H4431, Starter Kit, | 订制甲基纤维素培养基H4431, Starter Kit | 1 Custom | 19,233 | ||
| 不含细胞因子的培养基 | 04003 | MethoCult Z4003, 80 mL | 甲基纤维素培养基MethoCult Z4003, 80 mL | 检测体外药物敏感性，适用于人，小鼠，大鼠，狗 | 80 mL | 4,950 |
| 04534 | MethoCult H4534 Classic w/out EPO,100mL | 甲基纤维素培养基MethoCult H4534 Classic,100mL | 支持粒系集落，不支持红系集落 |
Free from STEMCELL Tutorial: Hematopoietic colonies derived from bone marrow Tutorial: Hematopoietic colonies derived from cord bloodRelated Products
 | MethoCult™ H4034 Optimum Methylcellulose-based Medium with Recombinant Cytokines and ... |
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 | MethoCult™ H4035 Optimum without EPO Methylcellulose-based Medium with Recombinant Cytokines (wit... |
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 | MethoCult™ H4435 Enriched Methylcellulose Medium with Recombinant Cytokines and EPO fo... |
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| MethoCult™ H4534 Classic without EPO (04534) | |
| 100 mL Request pricing | |
| MethoCult™ H4534 Classic without EPO (04544) | |
| 24 x 3 mL Request pricing | |
MethoCult™ H4534 Classic without EPO (MethoCult™ GF H4534) is optimized for the detection and quantification of human hematopoietic progenitors in bone marrow (BM), mobilized peripheral blood (MPB), peripheral blood (PB) and cord blood (CB) samples using colony-forming cell (CFC) assays. It is suitable for CD34+-enriched cells, mononuclear cells and cells isolated by other purification methods.MethoCult™ H4534 Classic without EPO is formulated to support optimal growth of granulocyte/macrophage progenitors (CFU-GM, CFU-G, CFU-M).
| Application: | colony assays & quantification |
| Product Type: | Specialized cell culture media |
| Recommended For: | colony-assays of human clonogenic hematopoietic progenitor cells in bone marrow, peripheral blood, cord blood, leukapheresis products and purified CD34+ cells |
| Accessory Products: | • Iscove's Modified Dulbecco's Medium (IMDM) with 2% Fetal Bovine Serum (Catalog #07700) • Blunt-end Needles (Catalog #28110/28120) • 3 cc Syringe (Catalog #28230/28240) • 35 mm Culture Dishes (Catalog #27100/27150) • 60 mm Gridded Scoring Dishes (Catalog #27500) |
| Contains: | • Methylcellulose in Iscove's MDM • Fetal Bovine Serum • Bovine Serum Albumin • 2-Mercaptoethanol • rh Stem Cell Factor • rh GM-CSF • rh IL-3 • Supplements |
| Area of Interest: | Cancer, Cord blood banking, Hematologic malignancies, Hematopoietic stem cell research, Stem cell biology, Transplantation |
| Cell Type: | Hematopoietic stem & progenitor cells |
| Medium Type: | Methylcellulose-based |
| Popular Product Line: | MethoCult™ |
| Species: | Human |
| Intended Use Statement: | FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. |
Protocols and Manuals
 Current documents |
| Product Name | Lot #  | document Type | Download |
| MethoCult™ H4534 Classic without EPO | All | Manual |  |
| MethoCult™ H4534 Classic without EPO | All | Product Information Sheet | |
MSDS
04534_04544-MSDS.pdfEducational Resources
VIDEOS & WEBINARS Procedure for Setting Up the CFC AssayBROCHURES Your Ideas. Our Tools. Products for every step of your hematopoietic stem and progenitor cell research. MethoCult™ for Hematopoietic colony-Forming Cell AssaysTECHNICAL BULLETINS Red Blood Cell Clearance Protocol The CFC Assay For Cord BloodFAQS
Q. WHY USE SEMI-SOLID MEDIA?
A. Semi-solid media (such as methylcellulose-based or collagen-based) allow the clonal progeny of a single progenitor cell to stay together so you can recognize distinct colonies. Q. WHY USE METHYLCELLULOSE-baseD MEDIA?
A. Methylcellulose is now widely used as a gelling agent because it permits better growth of erythroid colonies than other types of semi-solid support systems (e.g. agar) while allowing optimal granulocyte/macrophage colony formation. Committed progenitors for both erythroid and granulocyte/macrophage lineages, as well as multi-potential progenitors, can therefore be assayed simultaneously in the same culture dish. Q. IS IT NECESSARY TO ADD ANTIBIOTICS TO THE MEDIA?
A. No, we do not use antibiotics in our lab for colony assays. Aseptic technique should suffice. However, Penicillin/Streptomycin and an anti-fungal drug like amphotericin B can be added to the methylcellulose. The media should be mixed well prior to the addition of cells. Presence of antibiotics does not inhibit progenitor growth. Q. IS THERE ANYTHING I CAN DO IF MY PLATES ARE CONTAMINATED?
A. No, once contamination is visible, it is not possible to rescue the cultures by the addition of antibiotics. The bacteria/yeast inhibit colony formation by depleting nutrients or releasing toxic substances. Q. WHY CAN'T I USE A PIPETTE TO DISPENSE METHYLCELLULOSE-baseD MEDIA?
A. Because methylcellulose is a viscous solution and cannot be accurately dispensed using pipettes due to adherence of the medium to the walls of the pipette. 16-gauge (blunt-end) needles are recommended for accurate dispensing. Q. CAN I 'PLUCK' THE colonIES?
A. Yes, colonies can be 'plucked' using a pipettor and 200 µL sterile pipette tips or glass Pasteur pipette with an elongated tip. Colonies should be placed in a volume of 25 - 50 µL and diluted into suitable culture medium. Q. IS THERE A METHOCULT™ FORMULATION SUITABLE FOR HPP-CFC (HIGH PROLIFERATIVE POTENTIAL colonY FORMING CELL)?
A. Yes, MethoCult™ H4535 can be used as HPP-CFC does not require EPO. The culture period is usually 28 days. Feeding the media is not required as the growth factors are present in excess in the media. As these colonies can be quite large, overplating can be a problem. It is recommended to plate two different concentrations. The standard concentration, as well as a lower concentration. Q. WHY ARE LOW ADHERENCE DISHES SO importANT?
A. Because adherent cells such as fibroblasts can cause inhibition of colony growth and obscure visualization of colonies. Q. CAN METHOCULT™ PRODUCTS BE USED FOR LYMPHOID PROGENITOR CFC ASSAYS?
A. Human lymphoid progenitors (B,NK, and T) seem to require stromal support for growth therefore cannot be grown in MethoCult™. Mouse pre-B clonogenic progenitors can be grown in MethoCult™ M3630. Q. IS IT POSSIBLE TO SET UP CFC ASSAYS IN A 24-WELL PLATE?
A. Yes, as long as the same ratio of cells is plated. Optimal plating concentration and the number of wells required to get an accurate estimation of CFC numbers may require optimization:
• The surface area of a 35 mm dish is ~9.5 cm2 and 1.9 cm2 for the 24 well plate. Q. CAN I STAIN THE COLonIES IN THE METHOCULT™?
A. Colonies can be stained but only if they are plucked from the dish. However, mouse erythroid colonies can be stained in the dish (Benzidine Staining Protocol). CollagenCult™ products are recommended for staining applications. The 3D matrix can be dehydrated and the colonies can then be fixed onto glass slides for immunohistochemical or enzymatic staining. Q. ARE THERE DIFFERENCES IN colonY MORPHOLOGY WITH SERUM-FREE MEDIA?
A. The serum containing media generally give better overall growth (especially CFU-GM colonies, as they will contain more cells) but not more colonies. There is no difference in total CFC’s between serum-free and conditioned or recombinant media. Q. CAN METHOCULT™ BE MADE WITH AN ALTERNATE base MEDIA?
A. Yes, this can be done as a 'Custom' media order. Please contact techsupport@stemcell.com for more information. Data
Procedure summary for hematopoietic CFC Assays
Examples of colonies derived from CFU-GM
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