概述
Sepax SEC键合固定相是采用的表面修饰技术，通过在高纯度具有良好机械稳定性的硅胶基质上，键合一层均匀的纳米厚度的中性亲水涂层而制备得到。由于采用可控的化学修饰技术，因此能确保柱与柱之间有着可靠的重现性。SEC填料采用化学键合技术，表面亲水涂层覆盖完全，因此不仅具有优异的稳定性，而且对蛋白等生物样品的非特异性吸附作用也非常小。精心设计的大孔体积可保证高的分离容量以及优异的分辨率。广泛应用于生物分子及水溶性聚合物的分离和检测。
SRT体积排阻色谱柱优点 大的孔体积，高的柱容量，高的柱效，良好的重现性 宽的pH范围（pH范围2-8.5） 寿命长，一般在700-1000针 低盐浓度洗脱，适合LC-MS分析 对于生物样品常见的PEG或者吐温80等物质无强吸附 高的蛋白回收率，且蛋白不易失活 极低的非特异性吸附 不会出现二聚体与单体出峰紊乱的现象 解决多维色谱分析应用应用领域
蛋白药物 （人血白蛋白、免疫球蛋白、HGH、EPO等） 多肽(Peptide) 水溶性多聚物（多糖、PEG） 抗生素（头孢类抗生素） 抗体（Antibodies）、疫苗（Vaccine） 病毒蛋白(Virus Protein)、细胞发酵液、DNA
技术参数
| 固定相 | SRT SEC-100 | SRT SEC-150 | SRT SEC-300 | SRT SEC-500 | SRT SEC-1000 | SRT SEC-2000 |
| 材料 | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica |
| 颗粒大小 | 5 mm | 5 mm | 5 mm | 5 mm | 5 mm | 5 mm |
| 孔径 (Å) | ~ 100 | ~ 150 | ~ 300 | ~ 500 | ~ 1,000 | ~ 2,000 |
| 蛋白分子量范围 | 100 - 100,000 | 500 - 150,000 | 5,000 – 1,250,000 | 15,000 - 5,000,000 | 50,000 - 7,500,000 | > 10,000,000 |
| 水溶性聚合物分子量范围 | 500-10,000 | 500-25,000 | 1,000-100,000 | 2,500-500,000 | 5,000-1,500,000 | 50,000->2,500,000 |
| pH稳定性 | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) |
| 反压 (psi for a 7.8x300 mm) | ~ 700 | ~ 700 | ~ 700 | ~700 | ~700 | ~700 |
| 压力 (psi) | ~ 4,500 | ~ 4,500 | ~ 3,500 | ~ 3,000 | ~ 3,000 | ~ 3,000 |
| 盐浓度范围 | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M |
| 使用温度 | ~ 80oC | ~ 80oC | ~ 80oC | ~ 80 oC | ~ 80 oC | ~ 80 oC |
| 流动相兼容性 | Aqueous and organic | Aqueous and organic | Aqueous and organic | Aqueous and organic | Aqueous and organic | Aqueous and organic |
订购信息Sepax SRT SEC-100
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT SEC-100 | 5um | 100 Å | 21.5x600mm | 215100-21560 |
| SRT SEC-100 | 5um | 100 Å | 21.5x300mm | 215100-21530 |
| SRT SEC-100 | 5um | 100 Å | 7.5x600mm | 215100-7560 |
| SRT SEC-100 | 5um | 100 Å | 7.8x300mm | 215100-7830 |
| SRT SEC-100 | 5um | 100 Å | 7.8x150mm | 215100-7815 |
| SRT SEC-100 | 5um | 100 Å | 7.8x50 (Guard) | 215100-7805 |
| SRT SEC-100 | 5um | 100 Å | 4.6x300mm | 215100-4630 |
| SRT SEC-100 | 5um | 100 Å | 4.6x150mm | 215100-4615 |
| SRT SEC-100 | 5um | 100 Å | 4.6x50 (Guard) | 215100-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT SEC-150 | 5um | 150 Å | 21.5x600mm | 215150-21560 |
| SRT SEC-150 | 5um | 150 Å | 21.5x300mm | 215150-21530 |
| SRT SEC-150 | 5um | 150 Å | 7.5x600mm | 215150-7560 |
| SRT SEC-150 | 5um | 150 Å | 7.8x300mm | 215150-7830 |
| SRT SEC-150 | 5um | 150 Å | 7.8x150mm | 215150-7815 |
| SRT SEC-150 | 5um | 150 Å | 7.8x50 (Guard) | 215150-7805 |
| SRT SEC-150 | 5um | 150 Å | 4.6x300mm | 215150-4630 |
| SRT SEC-150 | 5um | 150 Å | 4.6x150mm | 215150-4615 |
| SRT SEC-150 | 5um | 150 Å | 4.6x50 (Guard) | 215150-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT SEC-300 | 5um | 300 Å | 21.5x600mm | 215300-21560 |
| SRT SEC-300 | 5um | 300 Å | 21.5x300mm | 215300-21530 |
| SRT SEC-300 | 5um | 300 Å | 7.5x600mm | 215300-7560 |
| SRT SEC-300 | 5um | 300 Å | 7.8x300mm | 215300-7830 |
| SRT SEC-300 | 5um | 300 Å | 7.8x150mm | 215300-7815 |
| SRT SEC-300 | 5um | 300 Å | 7.8x50 (Guard) | 215300-7805 |
| SRT SEC-300 | 5um | 300 Å | 4.6x300mm | 215300-4630 |
| SRT SEC-300 | 5um | 300 Å | 4.6x150mm | 215300-4615 |
| SRT SEC-300 | 5um | 300 Å | 4.6x50 (Guard) | 215300-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT SEC-500 | 5um | 500 Å | 21.5x600mm | 215500-21560 |
| SRT SEC-500 | 5um | 500 Å | 21.5x300mm | 215500-21530 |
| SRT SEC-500 | 5um | 500 Å | 7.5x600mm | 215500-7560 |
| SRT SEC-500 | 5um | 500 Å | 7.8x300mm | 215500-7830 |
| SRT SEC-500 | 5um | 500 Å | 7.8x150mm | 215500-7815 |
| SRT SEC-500 | 5um | 500 Å | 7.8x50 (Guard) | 215500-7805 |
| SRT SEC-500 | 5um | 500 Å | 4.6x300mm | 215500-4630 |
| SRT SEC-500 | 5um | 500 Å | 4.6x150mm | 215500-4615 |
| SRT SEC-500 | 5um | 500 Å | 4.6x50 (Guard) | 215500-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT SEC-1000 | 5um | 1000 Å | 21.5x600mm | 215950-21560 |
| SRT SEC-1000 | 5um | 1000 Å | 21.5x300mm | 215950-21530 |
| SRT SEC-1000 | 5um | 1000 Å | 7.5x600mm | 215950-7560 |
| SRT SEC-1000 | 5um | 1000 Å | 7.8x300mm | 215950-7830 |
| SRT SEC-1000 | 5um | 1000 Å | 7.8x150mm | 215950-7815 |
| SRT SEC-1000 | 5um | 1000 Å | 7.8x50 (Guard) | 215950-7805 |
| SRT SEC-1000 | 5um | 1000 Å | 4.6x300mm | 215950-4630 |
| SRT SEC-1000 | 5um | 1000 Å | 4.6x150mm | 215950-4615 |
| SRT SEC-1000 | 5um | 1000 Å | 4.6x50 (Guard) | 215950-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT SEC-2000 | 5um | 2000 Å | 21.5x600mm | 215980-21560 |
| SRT SEC-2000 | 5um | 2000 Å | 21.5x300mm | 215980-21530 |
| SRT SEC-2000 | 5um | 2000 Å | 7.5x600mm | 215980-7560 |
| SRT SEC-2000 | 5um | 2000 Å | 7.8x300mm | 215980-7830 |
| SRT SEC-2000 | 5um | 2000 Å | 7.8x150mm | 215980-7815 |
| SRT SEC-2000 | 5um | 2000 Å | 7.8x50 (Guard) | 215980-7805 |
| SRT SEC-2000 | 5um | 2000 Å | 4.6x300mm | 215980-4630 |
| SRT SEC-2000 | 5um | 2000 Å | 4.6x150mm | 215980-4615 |
| SRT SEC-2000 | 5um | 2000 Å | 4.6x50 (Guard) | 215980-4605 |
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