2021年12月9日，据欧盟食品安全局（EFSA）消息，应欧盟委员会要求，欧盟动物饲料添加剂和产品（FEEDAP）研究小组就谷氨酸棒杆菌KCCM 80185生产的L-异亮氨酸（l-isoleucine）作为所有动物饲料添加剂的安全性和有效性发表科学意见。
经过评估，专家小组认为在拟议的使用条件下，该添加剂对目标物种、消费者和环境是安全的。评估中的添加剂被认为是非反刍动物物种氨基酸L-异亮氨酸的有效来源。为了使补充的L-异亮氨酸对反刍动物和非反刍动物同样有效，需要防止其在瘤胃中降解。部分原文报道如下：
Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of a feed additive consisting of l-isoleucine produced by Corynebacterium glutamicum KCCM 80185 when used as a nutritional additive in feed and water for drinking for all animal species. The production strain is genetically modified, does not carry acquired antimicrobial resistance genes and no viable cells of the production strain were detected in the final product. The FEEDAP Panel could not exclude the presence of recombinant DNA from the production strain in the product. However, since no sequences of concern remain in the final production strain, the potential presence of recombinant DNA in the final product does not raise any safety concerns. The Panel concluded that the additive is safe for the target species, for the consumer and for the environment under the proposed conditions of use. Regarding the use in water, the FEEDAP Panel reiterated its concerns over the safety of l-isoleucine administered simultaneously via water for drinking and feed owing to the risk of nutritional imbalances and hygienic reasons. The FEEDAP Panel concluded that l-isoleucine produced by C. glutamicum KCCM 80185 is considered not toxic by inhalation, not irritant to skin or eyes and not a dermal sensitiser. However, due to the high dusting potential, exposure to dust per se might be a hazard for the user. l-Isoleucine produced by C. glutamicum KCCM 80185 is considered as an efficacious source of the essential amino acid l-isoleucine for non-ruminant animal species. For the supplemental l-isoleucine to be as efficacious in ruminants as in non-ruminant species, it would require protection against degradation in the rumen.
